If anyone's played Bioshock...

[Steve the Pocket]

...you'll know that the Little Sisters stop actually being as creepy as they're probably supposed to pretty early on in the game. Maybe this will help.

Added bonus: coincidentally, the typeface I used for the caption is a font called Love Ya Like A Sister.

[A dude named Vince]

I remember seeing the Little Sisters for the first time, and being only slightly less scared of them then the Big Daddies. I never really even played the game for more than an hour at a time, and I heard it got less scary as you went along (One game that didn't do that was Doom 3 *shudder*). Still, nice cartoony interpertation of the Little Sister, it really convey's their character while remaining somehow cartoonishly cute.

[The MAZZTer]

Don't Little Sisters have a more "zombie-ish" look? Nice never-the-less.

[MuffinSticks]

They didn't scare me. I ripped those fuсkin worm things right outta their... brains?.. Stomachs?.. Colons?

[The MAZZTer]

I rescue them all, your heartless beast!

(Not like it's a hard decision, you get rewards for doing it periodically which pretty much even out with the extra adam you'd get otherwise.)

[Baconsticks]

First time I played it through, I sacrificed all the little sisters. I had a shitload of ADAM.

(second time though, I saved them all)

[MuffinSticks]

Nah, you get 20 more adam (IIRC) if you sacrifice them as opposed to saving them and taking the extra adam from the teddy bear. The plasmids in the teddy bears suck balls.

[Steve the Pocket]

280 ADAM in all, according to the fan wiki -- not even enough to buy the level 3 Incinerate and Winter Blast plasmids. Once I realized the Chemical Thrower does both of those jobs effectively, and you practically trip over ammo for it wherever you go, I was able to get through without ever using those plasmids.

If anything, I don't think there's a big enough gameplay difference based on the Little Sisters. I would have suggested that the "good" route favor a less brute-force, more patient approach to winning, by giving you significantly less ADAM but providing tonics like Scrounger, Photographer's Eye, and Prolific Inventor.

I've heard this was one of the major complaints they received and one of the big things they're trying to correct in the sequel. I wish them luck.